 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Staphylococcus pseudintermedius isolation rate in our hospital and criteria for its accurate identification using biochemical tests |
| Language |
J |
| AuthorList |
Junpei Sasaki1), Shinobu Ishigaki1), Mitsuru Matsumura2), Miwa Asahara1), Yoshiko Atsukawa1), Lisa Ichikawa1), Yuta Kamimura1), Yasuo Ono3), Taiji Furukawa4) |
| Affiliation |
1) Department of Clinical Laboratory, Teikyo University Hospital
2) Department of Clinical Laboratory Science, Faculty of Medical Technology, Teikyo University
3) Department of Microbiology & Immunology, Teikyo University School of Medicine
4) Department of Laboratory Medicine, Teikyo University School of Medicine |
| Publication |
J.J.C.M.: 29 (3), 140-145, 2019 |
| Received |
November 8, 2018 |
| Accepted |
March 14, 2019 |
| Abstract |
Correct identification of Staphylococcus pseudintermedius using a bacterial identification automatic analyzers is difficult. In fact, its misidentification as Staphylococcus aureus or Staphylococcus intermedius has been reported. In this study, we determined the isolation rate of S. pseudintermedius in our hospital and established criteria for distinguishing S. pseudintermedius from other staphylococci based on biochemical characteristics. The isolation rate of S. pseudintermedius using VITEK MS from April 2015 to October 2016 was 0.1% (4/3,737 strains). Furthermore, when 8 strains previously identified as S. intermedius using Microscan WalkAway 96 were reanalyzed using VITEK MS, 1 was identified as S. pseudintermedius. The 5 strains identified as S. pseudintermedius using VITEK MS matched those identified using 16S rRNA gene sequencing and PCR method targeting thermostable nuclease (nuc) gene. When these 5 S. pseudintermedius strains were analyzed by MicroScan WalkAway 96, all were misidentified as S. intermedius. Comparison of the biochemical characteristics of the various species revealed that S. pseudintermedius was negative for the latex reagent for clumping factor, but positive for slow mannitol fermentation and high DNase production. These findings suggest that it is possible to correctly identify S. pseudintermedius based on the above biochemical characteristics, even in laboratories that use MicroScan WalkAway 96 without matrix-assisted laser desorption ionization time-of-flight mass spectrometry. |
| Keywords |
Staphylococcus pseudintermedius |
|
