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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Rapid detection of clarithromycin inducible resistance and determination of the antimicrobial susceptibility in clinical Mycobacteroides abscessus complex isolates |
| Language |
J |
| AuthorList |
Mika Kihara1), Motohisa Tomita1), Shiomi Yoshida2) |
| Affiliation |
1) Department of Clinical Laboratory, National Hospital Organization Kinki-chuo Chest Medical Center
2) Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center |
| Publication |
J.J.C.M.: 29 (4), 196-202, 2019 |
| Received |
January 23, 2019 |
| Accepted |
May 9, 2019 |
| Abstract |
Mycobacteroides abscessus complex (MABC), including three subspecies-M. abscessus, M. massiliense, and M. bolletii-is resistant to a variety of antibiotics so limited treatment options are available. The susceptibility of these subspecies to antimicrobial agents depends in particular on the erm(41), which are potentially related to inducible clarithromycin (CAM) resistance. The purpose of this study was to carry out identification of these subspecies based multiple sequencing, and erm(41). All forty-nine MABC isolates were identified as M. abscessus and M. massiliense and these subspecies could be discriminated between based on their resistance to CAM, as determined by truncation or mutation of erm(41). Regard to macrolide, all tested isolates were inhibited at higher median MICs by azithromycin (AZM) than CAM. Combination in MICs between CAM and AZM assumed all inducible CAM resistance isolates. MABC isolates were low minimal inhibitory concentrations (MICs) to meropenem, moxifloxacin and ciprofloxacin but were low MICs to imipenem and sitafloxacin. Our study demonstrates the importance of correct identification and antimicrobial susceptibility testing, including the testing of potential new agents, in the management of MABC infections. |
| Keywords |
Mycobacteroides abscessus, Mycobacteroides massiliense, erm（41） |
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