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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comparative study of the detection rates of the urinary antigen test and the LAMP method for Legionella species |
| Language |
J |
| AuthorList |
Wataru Shirasaka1), Masahiro Shinozaki1), Chieko Kushibiki2), Chihiro Katsukawa3) |
| Affiliation |
1) Department of Emergency and Critical Care Medicine, Kishiwada Tokushukai Hospital
2) Department of Clinical Laboratory Medicine, Kishiwada Tokushukai Hospital
3) Osaka Institute of Public Health, Division of Microbiology, Bacteriology Section |
| Publication |
J.J.C.M.: 29 (4), 203-206, 2019 |
| Received |
January 10, 2019 |
| Accepted |
May 16, 2019 |
| Abstract |
Legionella pneumonia is a severe disease and its early diagnosis and treatment are important. A laboratory test widely used for the rapid diagnosis of Legionella pneumonia is Urinary antigen test (UAT), but it is only detectable Legionellapneumophila serogroup 1. However, patients with a negative UAT result could be infected by Legionella pneumonia caused by other Legionella species and serogroups. Loop-mediated isothermal amplification (LAMP) method of sputum collected by endotracheal suction is an efficient, rapid, and accurate test for the detection of many species and serotypes of Legionella. From April 2012 to March 2018 (72 months), among the patients that visited the emergency department of our hospital, 667 patients were suspected of having Legionella pneumonia. From April 2012 to March 2015 (first 36 months), only three of 346 patients were found positive using UAT. From April 2015, when LAMP method was introduced, to March 2018 (next 36 months), 12 of 321 patients were found positive; LAMP method detected all the positive cases and UAT detected only four of the 12 cases. The detection rates of Legionella species were compared before and after the introduction of LAMP. The detection rate of LAMP method was 3.74%, significantly higher than the detection rate of UAT (0.87%) (P=0.0166). Therefore, we conclude that LAMP of sputum collected by endotracheal suction is a useful method for the detection of Legionella species and the rapid diagnosis of Legionella pneumonia. |
| Keywords |
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