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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Isolation and antimicrobial susceptibility of clinical isolates of nontuberculous mycobacteria analyzed in a clinical testing laboratory from 2011 to 2017 |
| Language |
J |
| AuthorList |
Takuya Tsunoda, Satomi Hashimoto, Shinichi Mashiba, Jun Ezoe, Satoshi Kugawa |
| Affiliation |
Health Science Research Institute, Inc. |
| Publication |
J.J.C.M.: 29 (4), 215-221, 2019 |
| Received |
November 5, 2018 |
| Accepted |
May 16, 2019 |
| Abstract |
We investigated the isolation and antimicrobial susceptibility of clinical isolates of nontuberculous mycobacteria (NTM) that were tested in our laboratory from January 2011 to December 2017. The specimens were mainly collected from small- and mid-sized medical facilities in Japan. The most detected NTM species by DNA-DNA hybridization with DDH Mycobacteria Kyokuto (Kyokuto Pharmaceutical Industrial Co., Ltd.) was Mycobacterium avium (M. avium) at 58.4% (n=7,967), followed by Mycobacterium intracellulare (M. intracellulare) at 17.2% (n=2,343), and Mycobacterium gordonae at 8.6% (n=1,171). The number of Mycobacteroides (Mycobacterium) abscessus and Mycolicibacterium (Mycobacterium) fortuitum has been increasing since 2011. Furthermore, the number of Mycobacteroides (Mycobacterium) chelonae, that had been increasing until 2015, has been decreasing after 2016. The MICs of streptomycin, ethambutol, kanamycin, rifampicin, rifabutin, levofloxacin, clarithromycin, ethionamide, and amikacin were determined by microdilution test with BrothMIC NTM (Kyokuto Pharmaceutical Industrial Co., Ltd.). Regarding the trend of antimicrobial drug resistance, there has been no significant positive change over time. Furthermore, the ratio of antimicrobial drug resistance and the MIC50 and MIC90 values of M. avium and M. intracellulare to clarithromycin and those of Mycobacterium kansasii to rifampicin were significantly low, indicating higher activity. This study was conducted only in vitro, so further studies are needed to evaluate the clinical effects of antimicrobial drugs. However, our epidemiological data should be useful for medical facilities, in particular small and mid-sized facilities, and for regional surveillance of NTM. |
| Keywords |
Nontuberculous mycobacteria（NTM）, DNA-DNA Hybridization（DDH）, BrothMIC NTM |
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