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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.30 No.1 contents] Japanese / English |
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| ArticleTitle | Comparative study using pulse field gel electrophoresis, Real time PCR, and PCR-based open reading frametyping kit for Acinetobacter species as typing methods of Acinetobacter sp. |
| Language | J |
| AuthorList | Mayuko Kaiho1,2), Takeshi Nakazawa2), Ayako Higuchi1), Yasuyo Ode1), Katsunari Kina1), Kazunori Miyake1), Kensuke Murata2,4), Yoshiaki Inoue5), Shinichi Sasaki2,3) |
| Affiliation |
1) Department of Clinical Laboratory, Juntendo University Urayasu Hospital 2) Division of Infection Control, Juntendo University Urayasu Hospital 3) Department of Pulmonology, Juntendo University Urayasu Hospital 4) Department of Emergency Room, Juntendo University Urayasu Hospital 5) Department of Emergency Room and Intensive Care Unit, Tsukuba University Hospital |
| Publication | J.J.C.M.: 30 (1), 18-24, 2019 |
| Received | August 9, 2018 |
| Accepted | October 2, 2019 |
| Abstract | Instance of Acinetobacter infection have increased in our institution since 2014. We thus attempted an epidemiological analysis using pulsed field gel electrophoresis (PFGE). Inspection by PFGE is cumbersome and costly and reporting results is time consuming. We investigated resistance genes of pandemic strains using real-time PCR for rapid differentiation of strains among Acinetobacter species, which could easily cause outbreaks. We also examined a PCR-based open reading frame typing (POT) kit for Acinetobacter species because it was newly marketed since 2014. We then compared the usefulness of these two methods based on the results of PFGE. We analyzed 92 strains that were determined as Acinetobacter species from June 2014 to October 2016. PFGE results revealed 30 strains to be the same clones (similarity≥90%) as they all showed the presence of ISAba1-blaOXA-51 by real-time PCR. Detecting a resistance gene by real-time PCR can distinguish a potential outbreak-causing strain; thus, it is a useful typing method for screening Acinetobacter species when their frequency of detection is increased. In the POT kit for Acinetobacter species, all 30 strains harboring ISAba1-blaOXA-51 were 122 by POT 1. According to the type of clones by POT 2 and POT 3, the strains were divided into 5 groups, which accounted for 122-24-55 (33%) and 122-26-55 (43%). Compared to PFGE, this method could identify the potential outbreak strains and simultaneously differentiate the same clones in a short time. However, it is impossible to detect resistance genes using POT kit for Acinetobacter species. |
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