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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.30 No.3 contents] Japanese / English |
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| ArticleTitle | Evaluation of the MASTDISCS combi Carba plus for the detection and classification of Carbapenemases in Enterobacterales. |
| Language | J |
| AuthorList | Masako Nishida1), Mari Kusuki1,2), Kenichiro Ohnuma1,3), Nami Ishida1), Saori Kobayashi1), Ai Ando1), Tatsuya Nakamura4), Goh Ohji1,2,5), Yuji Nakamachi1), Jun Saegusa1,3) |
| Affiliation |
1) Department of Clinical Laboratory, Kobe University Hospital 2) Department of Infection Prevention and Control, Kobe University Hospital 3) Division of Laboratory Medicine, Kobe University Graduate School of Medicine 4) Department of Medical Technology, Faculty of Health Sciences, Kyoto Tachibana University 5) Division of Infectious Diseases Therapeutics, Department of Microbiology and Infectious Diseases, Kobe University Graduate School of Medicine, Japan Department of Infection |
| Publication | J.J.C.M.: 30 (3), 127-134, 2020 |
| Received | December 14, 2019 |
| Accepted | March 25, 2020 |
| Abstract | The rapid spread of carbapenemase-producing Enterobacterales (CPE) has been one of the greatest concerns worldwide. Rapid and precise detection of the carbapenamase genotype may be useful for controlling nosocomial infections and selection of appropriate antibiotics. The purpose of this study was to evaluate the performance of the MASTDISCS combi Carba plus disc system (MD) in classifying the types of carbapenemases using 98 strains of Enterobacterales, including 46 strains of CPE. Except for GES-producers, the results of MD of 26 out of 39 isolates of MBL-producers, 2 isolates of OXA-48, and 1 out of 2 isolates of KPC were consistent with carbapenemase genotype. In particular, 11 out of 15 (73.3%) isolates harboring blaIMP-6 gene, a dominant carbapenemase genotype in Japan that tends to show low MICs to carbapenems, were correctly classified as MBL-producers. However, 4 isolates of MBL-producing Serratia marcescens and 1 isolate of Klebsiella pneumoniae harboring blaMOX gene were misidentified as OXA-48-producers and KPC-producer, respectively. Overall, concordance rates between MD and PCR was 84.2% (80/95). This study suggests that MD is a simple and inexpensive phenotypic testing system for the detection and differentiation of CPE as MBL-, KPC-, and OXA-48-producers in clinical microbiological laboratories. |
| Keywords | MASTDISCS combi Carba plus, faropenem |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |