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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
A case report of Vancomycin-dependent Enterococci from human sample in Japan |
| Language |
J |
| AuthorList |
Daiyu Kawamata1), Masakazu Sasaki2), Reika Sato1), Yuka Maebashi1), Hideaki Tachibana3), Tetsuo Yamaguchi4) |
| Affiliation |
1) Toda Central Medical Laboratory
2) Department of Clinical Laboratories, Toho University Omori Medical Center
3) Department of Dentistry, Showa University
4) Department of Microbiology and Infectious Disease, Toho University School of Medicine |
| Publication |
J.J.C.M.: 30 (3), 143-148, 2020 |
| Received |
December 14, 2019 |
| Accepted |
February 28, 2020 |
| Abstract |
We obtained vancomycin-dependent Enterococci (VDE) from clinical specimens, which can grow only with the presence of vancomycin. Since growth of the strain was not observed on normal agar medium plate without vancomycin, it was difficult to identify bacterial species and determine antimicrobial susceptibility. As a result of analysis, the strain was Vancomycin Resistant Enterococci (VRE) possessing vanB gene, and insertion sequence of 1,060 bp into ddl gene, which is responsible gene of D-Ala: D-Ala ligase required for D-alanyl-D-alanine terminal synthesis of peptidoglycan, was confirmed. It was considered that the strain that grew only in the presence of vancomycin was generated by the failure of D-Ala: D-Ala ligase. There are few reports worldwide, and it is a valuable case, and I think it will be useful for VRE detection methods including VDE in the future clinical laboratory field. |
| Keywords |
Vancomycin-dependent enterococci: VDE, VRE |
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