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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Detection of carbapenem-resistant Serratia marcescens harboring blaGES-5 |
| Language |
J |
| AuthorList |
Chiemi Fukuda1), Asuka Matsuda2), Mari Matsui3), Satowa Suzuki3), Tsuyoshi Sekizuka4), Makoto Kuroda4), Motoyuki Sugai3) |
| Affiliation |
1) Department of Microbiology, Kagawa Prefectural Research Institute for Environmental Sciences and Public Health
2) Clinical Laboratory, Takamatsu Red Cross Hospital
3) Antimicrobial Resistance Research Center, National Institute of Infectious Diseases
4) Pathogen Genomics Center, National Institute of Infectious Diseases |
| Publication |
J.J.C.M.: 31 (1), 17-21, 2020 |
| Received |
July 13, 2020 |
| Accepted |
September 9, 2020 |
| Abstract |
The increase and spread of bacterial multidrug resistance have emerged as global concerns. Herein, carbapenem-resistant Serratia marcescens was isolated from a urine sample of a 65-year-old male inpatient in Kagawa, Japan. The isolate was resistant to carbapenems (minimum inhibitory concentration [MIC] of imipenem, 4 μg/mL; meropenem, 32 μg/mL) but susceptible to broad-spectrum cephalosporins (MICs of ceftriaxone, ≤1 μg/mL; ceftazidime ≤4 μg/mL). The Carba NP test and modified carbapenem inactivation method indicated negative results. The combined-disc test using meropenem with 3-aminophenylboronic acid and with cloxacillin revealed positive and negative results, respectively. PCR screening for major carbapenemase genes including blaIMP, blaNDM, blaKPC, and blaOXA-48-like yielded negative results; however, positive results were obtained for blaGES. Whole-genome sequencing revealed that blaGES-5 is present in the class 1 integron (In1892) located on replicon type IncP(6) plasmid pMRY16-414SMA_2, having a total nucleotide length of 28,764 bp (GenBank Accession No. AY494717). It was challenging to determine whether the isolate produce the GES-type carbapenemase through phenotypic tests alone, and the genotypic test helped confirm these findings. This study highlights the need to monitor the less common type of carbapenemase, GES, along with the major carbapenemases. |
| Keywords |
GES-5, Carba NP test, Modified Carbapenem Inactivation Method (mCIM) |
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