 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Molecular epidemiological analysis of Mycobacterium tuberculosis strains isolated in Ishikawa Prefecture using the VNTR method |
| Language |
J |
| AuthorList |
Hiroe Kodama1), Megumi Kosaka1,2), Eriko Kimura1), Takayuki Shiomoto1), Emiko Kitagawa1,3), Mutsumi Tanimura1), Kazuhiko Izumo1), Keiko Kaneto1) |
| Affiliation |
1) Ishikawa Prefectural Institute of Public Health and Environmental Science
(2) Current address: Minami Kaga Health and Welfare Center of Ishikawa Prefecture)
(3) Current address: Health and Welfare Department of Ishikawa Prefecture) |
| Publication |
J.J.C.M.: 31 (1), 27-32, 2020 |
| Received |
January 28, 2020 |
| Accepted |
August 25, 2020 |
| Abstract |
We conducted a molecular epidemiological analysis of Mycobacterium tuberculosis strains isolated in Ishikawa Prefecture, Japan. We collected 170 strains and analyzed them using 12-locus variable number of tandem repeats (VNTR) (JATA12) and 24-locus VNTR (24Beijing). The Beijing genotype accounted for 73.5% of all the collected strains (125/170). The ratio that STK was detected in the strains isolated from the group of 70 years or older was significantly higher than the ratio of the group under 70 years. On the contrary, the ratio that modern Beijing was detected in the strains isolated from the group over 70 years old was significantly lower. Among the non-Beijing type, Euro-American was detected in 95% of the strains isolated from Japanese patients. Most of foreign-born patients were diagnosed with active tuberculosis within 2 years after their first visit to Japan, and the particular lineage of the strains isolated from them was different from the strains from Japanese patients. Therefore, it was estimated that most of the foreign-born patients had originally infected in their home countries. We applied the VNTR analysis using 24Beijing to 13 groups of 38 strains having the same profiles in JATA12. As a result, only two strains that were epidemiologically related each other had the completely same profile in 24Beijing. It was revealed that the VNTR analysis using 24Beijing was discriminatory method in precise determination of genetic characteristics of M. tuberculosis strain. |
| Keywords |
Mycobacterium tuberculosis, variable numbers of tandem repeats, JATA(12)-VNTR, 24Beijing-VNTR |
|
