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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.31 No.2 contents] Japanese / English |
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| ArticleTitle | Assessment of different storage conditions for Neisseria meningitidis survival |
| Language | J |
| AuthorList | Ayuka Kobayashi1), Jun Nakajima1), Takeshi Otsuka2), Masami Iigusa3), Yuichiro Sakai4), Akira Sata5), Takeya Ohshiro6), Koji Kikuchi7), Shun Kikuchi8), Yuko Matsumoto9), Seina Miyahara10), Masato Miyahira11), Hajime Kamiya12), Hideyuki Takahashi13), Shuji Tohda1), Ryoichi Saito14) |
| Affiliation |
1) Department of Clinical Laboratory, Tokyo Medical and Dental University, Medical Hospital 2) Department of Clinical Laboratory, Chiba Kaihin Municipal Hospital 3) Department of Infection Control and Prevention, Dokkyo Medical University Saitama Medical Center 4) Department of Clinical Laboratory, Yokohama City Minato Red Cross Hospital 5) Department of Clinical Laboratory, Miyazaki Prefectural Miyazaki Hospital 6) Department of Clinical Laboratory, Faculty of Medical Technology, Naha City Hospital 7) Clinical Microbiology Section, Division of Health Science, Saitama City Institute of Health Science and Research 8) Clinical Microbiology Section, Chiba Prefectural Institute of Public Health 9) Clinical Microbiology Section, Division of Inspection Research, Yokohama City Intitute of Health 10) Clinical Microbiology Section, Miyazaki Prefectural Institute for Public Health and Environment 11) Okinawa Prefecture Yaeyama Regional Public Health Center Public Environment Group 12) Infectious Disease Surveillance Center, National Institute of Infectious Diseases 13) Department of Bacteriology 1, National Institute of Infectious Diseases 14) Department of Molecular Microbiology Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University |
| Publication | J.J.C.M.: 31 (2), 108-112, 2021 |
| Received | July 13, 2020 |
| Accepted | December 3, 2020 |
| Abstract | Appropriate preservation of fastidious bacteria is required during transport because bacteria examination depends on the viability of bacteria in clinical specimens, which can be affected by conditions such as storage, shipment temperature and transport media. However, limited data are available on the storage conditions for keeping the survival of Neisseria meningitidis. In this study, we aimed to evaluate the ability of different transport media in maintaining the viability of N. meningitidis. Swabs inoculated with bacterial suspension of either N. meningitidis ATCC 13077 or clinical isolates (n=5) were stored in two transport media, Amies with charcoal and Cary Blair for 7 days at 4°C and 25°C. Both direct and quantitative methods were used to assess the number of viable cells. With the direct method, swabs were directly plated on chocolate agar followed by colony count. While with the quantitative method, swabs were first floated in sterile saline, a portion of the bacterial suspensions was then plated on chocolate agar, and colonies were counted. In both methods, the viability of ATCC 13077 kept in Amies with charcoal showed a milder time-dependent decrease at 4°C, compared to those stored at 25°C. Similar result was also obtained when Cary Blair was used; however, it was possible to be strain-dependent. Our findings indicated that the viability of N. meningitidis was maintained in both Amies with charcoal and Cary Blair and it will contribute to further understanding on the right condition for the shipment of N. meningitidis. |
| Keywords | Neisseria meningitidis |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |