 |
 |
 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
|
[Vol.13 No.1 contents] Japanese / English |
|
| ArticleTitle | Pulsed-Field Gel Electrophoresis Typing and PCR Ribotyping of Clostridium difficile Isolated in a University Teaching Hospital |
| Language | J |
| AuthorList | Etsuko Sawabe1,2), Anna Kakezuka2), Toshio Chida2), Kayo Osawa4), Noboru Okamura2), Isao Takebe1), Chino Sumita1), Hatsuko Toi1), Noriko Huruhata1), Masahiro Nishibori1), Nobuo Nara1), Shuji Miyake3), Yasuyuki Yoshizawa3) |
| Affiliation |
1) Department of Clinical Microbiology Laboratory, Tokyo Medical and Dental University Hospital 2) Department of Microbiology and Immunology, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University 3) Department of Pulmonary Medicine, Tokyo Medical and Dental University Hospital 4) Department of Medical Technology, Faculty of Health Sciences, Kobe University School of Medicine |
| Publication | J.J.C.M.: 13 (1), 1-7, 2003 |
| Received | October 8, 2002 |
| Accepted | March 4, 2003 |
| Abstract | Clostridium difficile strains isolated from patients hospitalized in the Tokyo Medical and Dental University Teaching Hospital from October 1999 to October 2000 were analyzed by three typing methods; pulsed-field gel electrophoresis (PFGE), PCR ribotyping and PCR toxigenicity. Fifty-five of the 59 isolates were classified into 23 types (A-W) with 34 electrophoretic patterns by PFGE, and all of the isolates were classified into 27 types (a-z, aa) by PCR ribotyping. In the ward in which 70% of C. difficile strains were isolated, the strains with PFGE type A and PCR ribotype a were the most frequently isolated and they were all positive for both toxin A and B. These results suggested that the nosocomial spread of C. difficile had occurred in the particular ward. |
| Keywords | C. difficile, PFGE |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |