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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Molecular Epidemiological Analysis and Antimicrobial Susceptibility of the Methicillin-Resistant Staphylococcus aureu s Strains Isolated in the University Teaching Hospital |
| Language |
J |
| AuthorList |
Toshio Chida1), Noboru Okamura1), Shizu Yoneyama1), Kayo Osawa2), Chiemi Baba1), Etsuko Sawabe1,3), Noriko Furuhata3), Hatsuko Toi3), Isao Takebe3), Chino Sumita3), Masahiro Nishibori3), Nobuo Nara3), Shuji Miyake4), Yasuyuki Yoshizawa4) |
| Affiliation |
1) Department of Microbiology and Immunology, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University
2) Department of Medical Technology, Faculty of Health Sciences, Kobe University School of Medicine
3) Department of Clinical Laboratory, Tokyo Medical and Dental University Hospital
4) Department of Geriatric and Pulmonary Medicine, Tokyo Medical and Dental University Hospital |
| Publication |
J.J.C.M.: 13 (1), 8-14, 2003 |
| Received |
October 1, 2002 |
| Accepted |
March 3, 2003 |
| Abstract |
The 185 strains of mechicillin-resistant Staphylococcus aureus (MRSA) isolated in the Tokyo Medical and Dental University Teaching Hospital from January to June in 2001 were examined for Sma I macrorestriction patterns of gemomic DNA by pulsed-field gel electrophoresis (PFGE) method, antimicrobial susceptibility by the agar dilution method and detection of toxic shock syndrome toxin (TSST-1) gene by the PCR method. Among 185 strains of MRSA, 79 PFGE patterns were detected, These PFGE patterns were classified into 16 (types a to p) DNA types by the Dice similarity index of more than 80%. The type i was the most common type (42%, 77 strains out of all MRSA strains). All of the isolates were sensitive to vancomycin and rifampin. Although we had never experienced arbekacin resistance before, 3 arbekacin-resistant strains were isolated for the first time in this hospital. TSST-1 gene was detected in 89% of the isolates. As for the spread of these strains over the hospital words, 26 strains with a particular PFGE pattern were distributed in the particular wards. Examination of these wards on patients' period of admission and excretion of MRSA suggested that cross-infection or colonization had occurred in these wards. Our results suggested that molecular epidemiological analysis of the MRSA combined with patients' clinical information was one of the effective means for warning the hospital staff of the possible cross-infection and keeping the patients from the outbreak of MRSA infection. |
| Keywords |
MRSA, PFGE, MIC |
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