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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Review for the research on 16S ribosomal RNA of its Single Stranded Regions |
| Language |
J |
| AuthorList |
Sadayori Hoshina |
| Affiliation |
Department of Laboratory Medicine, The Jikei University, School of Medicine |
| Publication |
J.J.C.M.: 14 (2), 79-91, 2004 |
| Received |
June 22, 2004 |
| Accepted |
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| Abstract |
Evolutionary phylogeny was accomplished by homology search for 16S ribosomal RNA sequences. This method has been used as bacterial identification system in laboratory medicine through hybridization technique and polymerase chain reaction. 16S rRNA is suitable gene target for identification of bacteria by its universal conserved sequence and its stable diversity of essential organelle as protein synthesis.
We could classify the ribosomal research to four areas in its early stage. First, its functional and structural analysis through conserved (single stranded) region of 16S ribosomal RNA sequence. Second, its usefulness of conserved sequences for detecting the bacteria. Third for evolutionary phylogeny study using 16S rRNA sequence homology. And fourth, identify the bacteria using 16S rRNA sequence diversity.
Research for ribosomal function through 16S rRNA single stranded region has been taken place in Europe and United States, especially Noller HF in California Santa Cruz. At the same time, Woese CR of Illinois University started to construct the evolutionary phylogeny using 16S rRNA double stranded region. Kone DE focused on detecting bacteria by this region.
Structural analysis for 16S rRNA was conducted in recently, suggested the model of computer assist structural biology. Function of these single stranded region was cleared for protein synthesis as peptidyl transferase center. These finding suggested the mechanisms of protein translation of rRNA was more applied method to genetic engineering of medicine. |
| Keywords |
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