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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Identification of VanD type Enterococcus raffinosus which has acquired a high-level resistance to vancomycin from a patients with long-term haemodialysis |
| Language |
J |
| AuthorList |
Hideyuki Maruyama1), Koichi Tanimoto2), Naohiro Shibata3), Harushige Kanno**4), Yasuyoshi Ike2) and Yoshichika Arakawa3) |
| Affiliation |
1) Icihara Hospital, Teikyo University School of Medicine (*Current address: Saiseikai Narashino Hospital, Chiba)
2) Department of Microbiology, Gunma University School of Medicine
3) Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases
4) Chiba University Hospital (**Current address: Takane Hospital) |
| Publication |
J.J.C.M.: 14 (2), 104-110, 2004 |
| Received |
February 3, 2004 |
| Accepted |
June 18, 2004 |
| Abstract |
Vancomycin resistant enterococci (VRE) which harbor vanA or vanB gene cluster have emerged and being proliferating in clinical settings located in Europe and North America. However, VRE strains belonging to Enterococcus faecalis and Enterococcus faecium which possess vanD gene are still very rare at present. In such circumstance, a VRE strain belonging to class D was isolated from an inpatient hospitalized in a Japanese clinical setting, and we characterized the feature of the VRE strain, as well as the clinical background of the patient. The patient has been suffered from diabetes mellitus and developed renal failure. He was administered with vancomycin after every haemodialysis for years to prevent bacterial infection. A strain of enterococci demonstrating a very high-level of resistance (MIC, > 256 μ g/ml) to both vancomycin and teicoplanin was isolated from gangrene at bed sore in his left lower extremity. This strain was identified as E.faffinosus by the conventional protocol of bacterial identification depend on mainly biochemical feature, although this strain could not utilize raffinose. Therefore, the nucleotide sequence of 16S rRNA was checked to confirm the species of this strain. As a conclusion, this strain was identified as E. raffinosus and found to harbor a gene closely related to vanD4 by a sequencing analysis of the PCR product amplified with PCR primers for detection of the genetic determinants for bacterial D-Ala-DAla ligases. |
| Keywords |
Enterococcus raffinosus, vanD4, VRE |
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