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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Studies of Clinical Microbiology and Molecular Biology of Staphylococcus aureus Isolated from Blood Culture and Non-blood Specimens of the Same Patients |
| Language |
J |
| AuthorList |
Shoji Moriki1), Akiko Nobata1), Hiroshi Shibata1), Junichi Masuda2) and Shunichi Kumakura3) |
| Affiliation |
1) Shimane University Hospital Central Clinical Laboratory
2) Shimane University School of Medicine Laboratory Medicine and Central Clinical Laboratory
3) Shimane University Hospital Division of Blood Transfusion |
| Publication |
J.J.C.M.: 14 (3), 153-158, 2004 |
| Received |
April 12, 2004 |
| Accepted |
August 16, 2004 |
| Abstract |
From July to December 2002, we found 7 bacteremic patients who were positive for Staphylococcus aureus in both blood and non-blood isolates. In total, 20 isolates were studied to examine the phenotypic and genetic relationship between blood and non-blood isolates in each patient. Pattern of antibiograms and the coagulase types of S. aureus from blood specimens were consistent with those from the non-blood specimens in each patient. Two patients had MSSA, out of which borderline-resistant S. aureus was identified in 1 case, and 5 patients had MRSA. Genetic analysis by pulsed-field gel electrophoresis (PFGE) showed the same patterns between blood and non-blood specimens in individual cases.
However, in 1 case, different PFGE patterns were observed between 2 blood isolates that were cultured under aerobic and anaerobic conditions. Our present results indicate that S. aureus strains obtained from blood and non-blood isolates were phenotypically and genetically identical in each patient, suggesting the possibility that the same clone of S. aureus could be systemically expanded in cases of bacteremia caused by this pathogen. |
| Keywords |
blood culture, borderline-resistant S. aureus, PCR (polymerase chain reaction), PFGE (pulsed-field gel electrophoresis), contact transmission |
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