 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Proposal of criteria for determining clinically significant bacteria using the time to positive culture of coagulase-negative staphylococci in blood cultures |
| Language |
J |
| AuthorList |
Takeya Ohshiro1,2), Kaoru Uchima1), Ryouko Ohshiro1), Orie Ohshiro1), Hideji Taira1), Kouhachi Oyakawa1) |
| Affiliation |
1) Department of Clinical Laboratory, Northern part of Okinawa Medical Association Hospital
2) Present address for corresponding: Department of Clinical Laboratory, Urasoe General Hospital |
| Publication |
J.J.C.M.: 14 (3), 177-182, 2004 |
| Received |
April 26, 2004 |
| Accepted |
September 27, 2004 |
| Abstract |
Ninety-three strains of coagulase-negative staphylococci (CNS) were used to determine the criteria for determining clinically significant bacteria based on the time to positive culture, positive slime production, and the presence of an indwelling central venous catheter. Blood culture was tested using BACTEC 9120 system, and slime-forming ability was tested on Congo red agar plates. Twenty-one strains (22.6%) were judged to be clinically significant bacteria, and the mean time to positive culture was 22.1 hours, which was significantly shorter than that (45.6 hours) to positive culture of contaminants (P< 0.0001). Twelve of the 13 slime-producing strains were determined to be significant bacteria, suggesting that slime-forming ability was useful for determining significant bacteria; however, the detection took at least 2 days. On the other hand, 15 (71.4%) of the significant bacteria were derived from patients with an indwelling central venous catheter, and were detectable in a significantly shorter time. Therefore, we propose that the criteria for determining clinically significant CNS detected from blood cultures should be the presence of an indwelling central venous catheter and the time to positive culture of less than 30 hours. Thirteen of the 15 strains meeting these criteria were determined to be significant bacteria, although there were some false-positives. Thus, the proposed criteria are clinically very reliable, fast, and easy to implement. |
| Keywords |
coagulase-negative staphylococci |
|
