 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of an Immunochromatographic Test for a Rapid Detection of Vero Toxins |
| Language |
J |
| AuthorList |
Ryuji Kawahara, Kazuko Seto, Masumi Taguchi, Kazuhiro Kobayashi |
| Affiliation |
Osaka Prefectural Institute of Public Health |
| Publication |
J.J.C.M.: 14 (3), 194-198, 2004 |
| Received |
July 8, 2004 |
| Accepted |
September 24, 2004 |
| Abstract |
Vero Toxins (VT) produced by Enterohemorrhagic Escherichia coli (EHEC) are one of the major virulence factors and bring lethal outcome to some patients due to hemolytic uremic syndrome. CapiliaVT, an immunochromatographic test for the rapid detection of the VT, was evaluated by comparison with the reversed passive latex agglutination (RPLA) kit. All specimens prepared with EHEC isolated 39 strains showed positive and all other pathogenic bacteria 50 strains showed negative for the CapiliaVT kit. The results indicated that the specificity of the kit was completely corresponding with that of RPLA kit. Though, the sensitivity was lower than that of RPLA (between 1/4 to 1/16) when dilution test of samples obtained with EHEC isolates was performed. The sensitivity and specificity of the CapiliaVT kit was lower than that of conventional culture method (EHEC isolation) when 230 stool specimens and 83 further cultured broth were examined. It was shown that the CapiliaVT kit needs further improvement for detection VT in clinical specimens. Considering its rapidity and simplicity, we conclude that the CapiliaVT is the excellent kit to identify EHEC for the isolated strain but remain a problem with sensitivity and specificity. |
| Keywords |
|
|
