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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Loop-mediated Isothermal Amplification (LAMP) Method: A Novel Nucleic Acid Amplification Method |
| Language |
J |
| AuthorList |
Masanari Ikedo |
| Affiliation |
Biochemical Research Laboratory, Eiken Chemical Co., Ltd. |
| Publication |
J.J.C.M.: 15 (1), 1-8, 2005 |
| Received |
February 21, 2005 |
| Accepted |
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| Abstract |
A novel DNA amplification method, the loop mediated isothermal amplification (LAMP) method was established in 2000. This method has the characteristic of high specificity, sensitivity, rapidity and simple procedure. Polymerase chain reaction (PCR) method is generally used as a rapid diagnosis procedure in the field of clinical microbiology. But, it needs the expensive reagents and instruments, and high-level knowledge and technique to the personnel for operation, so it would be difficult to use in the clinical laboratory. The LAMP is able to perform under isothermal condition with simple procedure and to obtain the results in less than one hour without particular instrument. Because of these advantages, the LAMP method would be use in clinical laboratory as a practical method for diagnosis of infectious diseases. |
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