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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of an Immunochromatographic Assay Kit for a Rapid Detection of Enterohemorrhagic Escherichia coli O157 |
| Language |
J |
| AuthorList |
Kazuko Seto, Ryuji Kawahara, Masumi Taguchi, Kazuhiro Kobayashi |
| Affiliation |
Osaka Prefectural Institute of Public Health |
| Publication |
J.J.C.M.: 15 (1), 20-24, 2005 |
| Received |
July 8, 2004 |
| Accepted |
January 12, 2005 |
| Abstract |
The clinical utility of a new immunochromatographic assay kit Capilia O157 for diagnosis of enterohemorrhagic Escherichia coli O157 (EHEC O157) infection was compared with that of the commercial kit NOW EH E. coli O157 (NOW). When stool specimens from 112 culture-positive persons were directly tested with Capilia O157 and NOW, 100 and 96 samples gave positive results, respectively. Both kit showed false-positive results in seven and two stool specimens from 168 culture-negative persons, respectively. Sensitivity of Capilia O157 was determined using the pure cultures of 42 clinical isolates possessing O157 antigen. Capilia O157 detected them at levels approximately 10-fold lower than those gave positive results with NOW. These results indicated that Capilia O157 was useful as a screening tool for detecting EHEC O157 in stool specimens. |
| Keywords |
O157 |
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