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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
A Detection Method of Cereulide, an Emetic Toxin of Bacillus cereus, Using High-Performance Liquid Chromatography-Mass Spectrometry |
| Language |
J |
| AuthorList |
Kumiko Kawamura1), Yumi Hirama1), Norio Agata2), Hideo Ito1), Michio Ohta3) |
| Affiliation |
1) Department of Medical Technology, Nagoya University School of Health
Sciences, 1-1-20 Daikominami, Higashi-ku, Nagoya 461-8673
2) Nagoya City Public Health Research Institute, 1-11 Hagiyama-cho, Mizuho-ku, Nagoya 467-8615
3) Department of Bacteriology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 |
| Publication |
J.J.C.M.: 15 (2), 68-76, 2005 |
| Received |
November 11, 2004 |
| Accepted |
March 16, 2005 |
| Abstract |
This paper describes a sensitive and specific chemical assay for cereulide, the heat-stable emetic toxin, produced by Bacillus cereus. The previous method for measuring cereulide is a bioassay that gives a cytotoxicity (HEp-2 cell vacuolation) titer, but not an accurate concentration and requires a specific skill. We developed a sensitive and specific chemical assay for cereulide based on high-performance liquid chromatography (HPLC)-mass spectrometry (MS). This method was calibrated with the purified cereulide and valinomycin as an internal control and a structurally similar cyclic depsipeptide. The detection limit for cereulide by this methos was 1.0 pmol and this method gave constant results over a wide range of cereulide concentrations, ranging from 0.1 to 25.0 μ g/mL. Therefore, this method enabled the quantification of cereulide in this range of concentration. The results obtained by this method were in good agreement with those of the HEp-2 cell vacuolation test. This method is reproducible and so sensitive and specific that it can be applied for detecting a trace amount of cereulide in causative foods. |
| Keywords |
Bacillus cereus, HPLC-MS |
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