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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Study of 4 Isolates Being Required to Differentiate Mycobacterium tuberculosis from Mycobacterium bovis Bacillus Calmette-Guerine (BCG) |
| Language |
J |
| AuthorList |
Ryoko Goto1,2), Tetsuro Muratani1,3), Tomoko Kobayashi1,2), Chieko Ikeura2), Shizuka Lee H.1,3), Yuko Odahara1), Tetsuro Matsumoto3) |
| Affiliation |
1) Hibiki Research Group for Clinical Microbiology
2) Kyurin Corporation
3) Department of Urology, School of Medicine, University of Occupational and Environmental Health |
| Publication |
J.J.C.M.: 15 (3), 120-125, 2005 |
| Received |
April 13, 2005 |
| Accepted |
August 17, 2005 |
| Abstract |
We experienced 4 cases being required to differentiate Mycobacterium tuberculosis from Mycobacterium bovis bacillus Calmette-Guerine (BCG). All isolates (MYC1, MYC2, MYC3, and MYC5) from 4 cases showed PCR-positive results when using Cobas Amplicor Mycobacterium tuberculosis. The results of "Differentiation medium set for Mycobacteria" (Kyokuto Pharmaceuticals) against 4 isolates were tubercle bacillus. Mycobacterium bovis could be denied because these 4 strains showed the results of niacin production-positive and nitrate reductase-positive. We used 10 μ g/ml of thiophene-2-carboxylic acid hydrazide (TCH) to differentiate M. tuberculosis from BCG. MYC1 and MYC2 strains were not growth on Ogawa medium containing TCH, but MYC3 and MYC5 strains grew on the same medium. These biochemical results showed that MYC1 and MYC2 strains are BCG and MYC3 and MYC5 strains are M. tuberculosis. Furthermore we underwent PCR used PCR primer reported by Yamazaki et al. to differentiate M. tuberculosis from BCG. The result of the PCR against MYC1 and MYC2 strains were the same as it against M. bovis BCG Tokyo, it against MYC3 and MYC5 strains was the same as it against M. tuberculosis.
The intravesical BCG instillation in the treatment of superficial bladder cancer has become general treatment. The various tubercle as adverse drug reaction caused by BCG-treatment has been reported. As BCG-treatment is increasing; we will experience more cases of tubercle caused by BCG after this. We hope the Ogawa medium containing 10 μ g of TCH will be obtainable at market to differentiate M. tuberculosis from BCG. |
| Keywords |
Mycobacterium, BCG, TCH |
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