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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
The Reproducibility of MIC Measured with BrothMIC NTM against Mycobacterium avium-M. intracellulare Complex |
| Language |
J |
| AuthorList |
Miki Goto1), Mayumi Ichikawa1), Masahiro Watanabe1), Shunsuke Shibuya1), Shigemi Hitomi2) |
| Affiliation |
1) Department of Clinical Laboratory, University of Tsukuba Hospital
2) Department of Infectious Diseases, Institute of Clinical Medicine |
| Publication |
J.J.C.M.: 16 (2), 67-73, 2006 |
| Received |
August 29, 2005 |
| Accepted |
February 9, 2006 |
| Abstract |
We examined the reproducibility of minimum inhibitory concentration (MIC) measured with BrothMIC NTM using six clinical isolates of Mycobacterium avium and six of Mycobacterium intracellulare. Three plates per isolate were inoculated and MIC were measured in each plate by two groups of examiners, either experienced or inexperienced ones in determining MIC with BrothMIC NTM, before and after guidance for MIC determination. The ranges of MICs measured by the experienced ones after guidance were more than fourfold in kanamycin against one M. avium and isoniazide against one M. intracellulare. The reproducibility of MIC by the experienced ones was better than that by the inexperienced ones in streptomycin, ethambutol, kanamycin, rifampicin, levofloxacin, clarithromycin, and ethionamide against M. avium and in streptomycin, ethambutol, kanamycin, and amikacin against M. intracellulare. The reproducibility by the inexperienced group improved only in ethambutol against M. avium after guidance. We conclude that prior trainings for optical measurement of MIC are indispensable to use BrothMIC NTM. |
| Keywords |
MAC, MIC |
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