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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.16 No.2 contents] Japanese / English |
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| ArticleTitle | Usefulness of RFLP Method for Identification of Recent Isolates of Nocardia spp. and Antimicrobial Susceptibility Testing |
| Language | J |
| AuthorList | Rie Shibuya1), Kazuhiro Tateda1,2), †, Soichiro Kimura1), Yoshikazu Ishii1), Hinako Murakami2), Reiko Shimatsu1), Fusako Kashitani1), Morihiro Iwata2), Tetsuya Matsumoto3), Kazuhiro Kimura4), Koh Uchida4), Koichiro Nakata4), Setsuko Kubo5), Yuzuru Mikami5), Keizo Yamaguchi1,2) |
| Affiliation |
1) Department of Microbiology and Infectious Diseases, Toho University School of Medicine; † Corresponding author 2) Clinical Laboratory, Toho University Omori Medical Center 3) Department of Microbiology, Tokyo Medical University 4) Respiratory Medicine, Internal Medicine, Toho Unviversity School of Medicine 5) Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba Univer-sity, Chuo-ku, Chiba 260-8673, Japan |
| Publication | J.J.C.M.: 16 (2), 81-88, 2006 |
| Received | February 8, 2006 |
| Accepted | April 19, 2006 |
| Abstract | In this study, we examined usefulness of RFLP method for identification of clinical isolates of Nocardia spp. (standard strains and clinical isolates), comparing to conventional biochemical assays. Twenty-six strains of clinical isolates were conventionally identified as N. asteroides (n=12), N. nova (n=8), N. farcinica (n=6). In contrast, RFLP method using bacterial 16S rDNA demonstrated N. asteroides (n=4), N. farcinica (n=4), N. transvalensis (n=4) in 12 strains of N. asteroides conventionally identified. Also RFLP results showed N. nova (n=2), N. farcinica (n=1), unclassified (n=5) in 8 strains of N. nova conventionally identified, whereas all 6 strains N. farcinica indicated identical results. Antimicrobial susceptibility testing showed that the first-line antibiotics, such as minocycline, amikacin, imipenem and ST, in addition to newer antimicrobials (linezolid, arbekacin), were generally active against clinical isolates of Nocardia spp., although a small variation was observed among strains and species. These data suggest that RFLP method for identification of Nocardia organisms may be a useful technique in the clinical microbiological laboratory setting. |
| Keywords | Nocardia, RFLP, conventional identification, antimicrobial susceptibility testing |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |