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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Phylogenetic and Drug Resistant Gene Analysis of Haemophilus influenzae from Bacterial Meningitis -Same Original or Different Specimen |
| Language |
J |
| AuthorList |
Akiko Nobata1), Syoji Moriki1), Hidehiko Moriyama1), Hiroshi Shibata1), Junichi Masuda2) |
| Affiliation |
1) Shimane University Hospital Central Clinical Laboratory
2) Shimane University School of Medicine Laboratory Medicine and Central Clinical Laboratory |
| Publication |
J.J.C.M.: 16 (3), 134-140, 2006 |
| Received |
September 26, 2005 |
| Accepted |
July 25, 2006 |
| Abstract |
We report phenotypic and genetic relationship of Haemophilus influenzae strains derived from cerebrospinal fluid (CSF), blood, and the nasopharynx in each patient of the 6 children with meningitis. We analyzed these isolates by serotyping, biotyping, antimicrobial susceptibility testing and PCR to identify resistant genes in H. influenzae. Case 2 had β -lactamase non-producing ABPC-resistant strain (BLNAR) and β -lactamase producing CVA/AMPC-resistant strain possessing TEM-1 and BLNAR-resistant genes (BLPACR-II) in the nasopharynx. The two strains exhibited same PFGE profiles. One patient had β -lactamase non-producing ampicillin (ABPC) susceptible strains (BLNAS), 2 patients had TEM-1 type β -lactamase producing ABPC resistant strains (BLPAR), 1 patient had β -lactamase producing clavulanic acid/amoxicillin (CVA/AMPC) resistant strains possessing TEM-1 and low-BLNAR resistant genes (BLPACR-I), and 1 patient had BLNAR. PFGE showed the same patterns among isolates from individual patients. Our results suggest that the same clone of H. influenzae could systematically expand casing bacterial meningitis. |
| Keywords |
H. influenzae type b (Hib), TEM-1 type β-lactamase, pulsed field gel electrophoresis (PFGE) |
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