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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
ArticleTitle |
Clinical Utility of Liquid Media on Screening in Pregnant Women for Vaginal Colonization of Group B Streptococcus |
Language |
J |
AuthorList |
Mizuho Iwasaki, Masaru Komatsu, Yoko Nagasaka, Saori Fukuda, Noriyuki Abe, Akihiro Nakamura |
Affiliation |
Department of Clinical Pathology, Tenri Hospital |
Publication |
J.J.C.M.: 16 (3), 141-144, 2006 |
Received |
April 6, 2006 |
Accepted |
August 16, 2006 |
Abstract |
Group B streptococcus (GBS) is a leading cause of such serious neonatal infections as meningitis and sepsis. The Centers for Disease Control and Prevention in the United States has recommended universal screening of all pregnant women at 35-37 weeks' gestation for GBS with the use of a selective broth medium. We compared an enrichement method using selective Todd-Hewitt broth with subculture to colistin-nalidixic acid blood agar to a direct culture method with 5% sheep blood agar. A total of 221 vaginal specimens submitted for detection of GBS were included in the study. Twenty-five GBS isolates (11%) were recovered from enriched culture and 12 GBS isolates (5%) were recovered from direct culture. All isolates that were detected in the direct culture were also detected in the enriched culture. These findings show that the enriched culture method with selective Todd-Hewitt broth is more sensitive than the direct culture method, and because it is more costeffective, it may became replaced as the only method used. |
Keywords |
group B streptococcus, liquid media, screening in pregnant women |
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