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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of CapiliaTB for Identification of Mycobacterium tuberculosis Complex, with Special Reference to the Culture Medium |
| Language |
J |
| AuthorList |
Akemi Kodama1), Hajime Saito2) |
| Affiliation |
1) Clinical Laboratory, National Hospital Organization, Higashi-hiroshima Medical Center
2) Hiroshimashi Environment and Health Association |
| Publication |
J.J.C.M.: 17 (2), 109-118, 2007 |
| Received |
October 25, 2006 |
| Accepted |
April 19, 2007 |
| Abstract |
CapiliaTB is a newly developed Mycobacterium tuberculosis complex identification assay based on immunochromatography utilizing anti-MPB64 monoclonal antiboby. Of 30 cultures tested on the day when BBL MGIT supplemented with MGIT OADC and MGIT PANTA, KRD "Nichibi," MYCOACID or 2% Ogawa medium which was inoculated with 0.1 ml of 104-105 CFU/ml of isolates from sputum with BACTEC MGIT 960 system became positive for acid-fast bacilli, 21 M. tuberculosis complex were strongly positive in MGIT, false positive to strongly positive in KRD and strongly positive in Ogawa for CapiliaTB, while no positive reaction was observed in MYCOACID. Of 141 laboratory stock cultures of 26 mycobacterial species grown in Middlebrook 7H9 broth, all 59 M. tuberculosis strains displayed positive reactions. Among nontuberculous mycobacteria, 59 strains of 22 species showed no positive signal and 6 of 6 Mycobacterium marinum, 16 of 20 Mycobacterium ulcerans and 1 of 15 Mycobacterium avium showed a false positive signal. None of M. marinum strains grown on 2% Ogawa medium showed a positive reaction for the CapiliaTB during the course of the experiment (21 days), while all strains grown on MGIT, MYCOACID or KRD medium eventually displayed a positive reaction which intensified with time. Among 173 cultures which were isolated from sputum on 2% Ogawa medium and stocked at room temperature for 3 weeks to 7 months, 126 M. tuberculosis complex were positive for CapiliaTB except for one strain and for both AccuProbe and the niacin accumulation, and 47 nontuberculosis mycobacteria showed no positive reactions. These results indicate that CapiliaTB is simple, rapid and reliable identification test for M. tuberculosis complex in liquid culture systems, especially with MGIT or a conventional solid medium such as 2% Ogawa medium. |
| Keywords |
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