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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
An Analysis of Methicillin-resistant Staphylococcus aureus Isolated in 7 Hospitals in Yokohama City |
| Language |
J |
| AuthorList |
Mikiko Yamada, Yuko Matsumoto, Yukiko Ishiguro, Harue Kitazume, Tetsunori Muto, Masanori Toba |
| Affiliation |
Yokohama City Institute of Health |
| Publication |
J.J.C.M.: 17 (2), 131-136, 2007 |
| Received |
October 25, 2006 |
| Accepted |
April 9, 2007 |
| Abstract |
An analysis of hospital-derived methicillin-resistant Staphylolcoccus aureus (MRSA) was conducted using 124 strains isolated in 7 hospitals in Yokohama city from 1997 to 2003. Although coagulase produced by 80 strains (65%) revealed to be type II, that of 35 isolates (28%) remained "untypable". As for toxins, among Staphylococcal Enterotoxin types AB, BC, B, and C which were observed in 106 strains (85%), the majority was type C (91; 73%), and 108 strains (87%) produced Toxic Shock Syndrome Toxin-1, while Exfoliative Toxin was detected only in 7 (5.6%) isolates. Analysis of PFGE patterns after digestion with restriction enzyme SmaI was done using 122 isolates. These were grouped into 83 clusters, and the Dice similarity coefficients of them scattered from 0.56 to 1.00. Patterns of CspI-digested DNA fragments were also compared in combination with the above results, and the fact of interest found out was that several groups having similarity coefficient 1.00 with either SmaI or CspI treatment were divided into 2 clusters when digested with the other enzyme. |
| Keywords |
MRSA, pulsed-field gel electrophoresis (PFGE), molecular epidemiology, epidemiological marker |
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