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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of Three Techniques for Detection of Low-Level Methicillin-Resistant Staphylococcus aureus (MRSA) |
| Language |
J |
| AuthorList |
Rie Mizuno1), Kumiko Kawamura1), Toshi Nada3), Hisashi Baba3,4), Hideo Ito1) |
| Affiliation |
1) Department of Medical Technology, Nagoya University School of Health Science
3) Department of Clinical Laboratory Medicine, Nagoya University Hospital
4) Department of Infectious Diseases, Nagoya University Hospital |
| Publication |
J.J.C.M.: 17 (3), 177-185, 2007 |
| Received |
May 11, 2007 |
| Accepted |
July 14, 2007 |
| Abstract |
Some of methicillin-resistant Staphylococcus aureus (MRSA) is often misdiagnosed as methicillin-susceptible S. aureus (MSSA) by the susceptibility testing. Although, detection of the mecA gene by PCR was considered to be the "gold standard," the susceptibility tests have been performed in the clinical laboratory. The aim of our study was to evaluate the performances of three methods for detection of MRSA and find more accurate and sensitive methods for detection of MRSA isolates. The 153 clinical isolates of S. aureus studied were divided into 99 MRSA (mecA-positive) and 54 MSSA (mecA-negative) isolates by PCR. The sensitivity of both the PBP2a production by latex agglutination test and the MRSA screening medium were 100%. The sensitivity of both broth microdilution method and the disk diffusion method with CFX were 97.0%, and those were superior to the disk diffusion method with MPIPC for recovery of MRSA. There were 15 isolates (low-level MRSA) that the results obtained by the susceptibility testing with MPIPC or CFX were different from those of PCR. The PBP2a expression of some low-level MRSA isolates by western blot assay were reduced, but the PBP2a production by latex agglutination test and the MRSA screening medium detected all 15 low-level MRSA isolates. Therefore, the results of our experiment confirmed usefulness of the susceptibility testing with CFX, the PBP2a production by latex agglutination test, and some sorts of the MRSA screening medium. We suggested that combination with these methods is the best performing tests for routine detection of all MRSA including low-level MRSA isolates. |
| Keywords |
Low-level MRSA |
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