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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Transcription-Reverse Transcription Concerted Reaction in Detecting Mycobacterium tuberculosis (TRCRapid M. TB) |
| Language |
J |
| AuthorList |
Taku Tamura, Kenji Tominaga, Kazumi Sakamoto, Harumi Tsurukawa, Saori Maeda, Mariko Wakabayashi, Mitsutaka Sasaki, Tomoko Ishikawa, Ikurou Abe, Ken Kaito |
| Affiliation |
Central Clinical Laboratory, Jikei University Hospital, 3-19-18 Nishishinbashi, Minato-ku, Tokyo 105-847, Japan |
| Publication |
J.J.C.M.: 18 (1), 15-19, 2008 |
| Received |
August 29, 2007 |
| Accepted |
January 17, 2008 |
| Abstract |
Transcription-reverse transcription concerted reaction system (TRC system) is a new method for the amplification and the detection of the target RNA, and it is recently introduced to the rapid detection system for Mycobacterium tuberculosis as TRCRapid M. TB (TOSOH). We examined the presence of Mycobacterium tuberculosis in 84 samples by TRC system, PCR system and liquid culture, and the efficiency of TRCRapid M. TB (TOSOH) was evaluated. As a result, the rate of agreement between TRC system and PCR system was 97.6%, and the rate between TRC system and liquid culture was 96.4%. The discrepancy was observed in a few samples, while it was disappeared by cryo-preservation of the applied samples. In such samples, the detection time of the internal control was also prolonged. Therefore, we could find the false negative phenomenon by paying attention to such prolongation. These results indicated that TRC system for Mycobacterium tuberculosis is a very satisfactory system, and could be introduced to the clinical use. |
| Keywords |
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