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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comparison of the Four Drug Susceptibility Testing Plates for Haemophilus influenzae, and Comparison with the Gene Mutations of Penicillin-Binding Protein 3 |
| Language |
J |
| AuthorList |
Chihiro Shimizu, Tatsuya Nakamura, Mayumi Kasahara, Kazuyuki Okuda, Chiyo Nakata, Hirorko Fujimoto, Hiroe Okura, Hakuo Takahashi |
| Affiliation |
Clinical Central Laboratory, Kansai Medical University Hirakata Hospital, 2-3-1 Shinmachi, Hirakata-shi, Osaka 573-1191, Japan |
| Publication |
J.J.C.M.: 18 (2), 90-99, 2008 |
| Received |
January 24, 2007 |
| Accepted |
April 18, 2008 |
| Abstract |
In drug susceptibility tests of Haemophilus influenzae (H. influenzae), Haemophilus test medium (HTM) and Mueller-Hinton broth (MHB) supplemented with 5% defribrinated horse blood are used. We compared performance levels among the 4 types of plates for the drug susceptibility test of H. influenzae on the market. For ampicillin, there was a good correlation (387%) between each type of measurement panel. The BLNAR detection rate was 18.0%, 19.7%, 39.3%, 37.7% by each panel. The correlation between the MIC of ABPC and gene mutation was evaluated, some BLNAR and ABPC-low resistant strains that did not grow in HTM were shown to be BLNAS by MHB. Since MIC differs among media and methods in the drug susceptibility test of H. influenzae, measurement results should be evaluated with the understanding of its characteristics. |
| Keywords |
Haemophilus influenzae, ampicillin, BLNAR, HTM, MHB |
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