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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
ArticleTitle |
An Autopsy Case of Infectious Aortic Aneurysm Caused by Nontyphoid Salmonella: PCR-Based Genus-Specifc invA Gene Detection Aided the Diagnosis |
Language |
J |
AuthorList |
Yuka Inoue, Makoto Ito, Masaru Okugawa, Hitoshi Kuramae, Natsuko Matsui, Yoshio Izawa, Akiyoshi Sakai |
Affiliation |
Department of Pathology and Laboratory Medicine, Kariya Toyota General Hospital |
Publication |
J.J.C.M.: 18 (2), 109-113, 2008 |
Received |
May 2, 2007 |
Accepted |
February 21, 2008 |
Abstract |
An autopsy case of infectious aortic aneurysm in a 62-year-old diabetic male patient was reported. The causative pathogen isolated from blood culture was identified as Salmonella enterica serovar, Enteritidis. To confirm a patho-etiologic relationship we attempted a PCR-based detection of nontyphoid Salmonella genus-specific invA gene from formalin-fixed paraffin sections. By using DNA templates extracted from tissue sections prepared from the aneurysmal site, conventional PCR protocol was able to detect the Salmonella-specific invA amplicon with the primer set designed to amplify a short flanking region (107-bp) within the open reading frame of invA. The detection of pathogen-specific genes from various resources may serve as a diagnostic adjunct for determining the etiology of deep-seated infections, even when there are solely autopsy materials available. |
Keywords |
nontyphoid Salmonella, PCR |
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