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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Identification of Mycobacterium tuberculosis Complex Strains in Kyushu University Hospital |
| Language |
J |
| AuthorList |
Yuiko Morokuma, Yujiro Uchida, Takahito Karashima, Masako Fujise, Shoichi Imamura, Yuzo Kayamori, Dongchon Kang |
| Affiliation |
Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital |
| Publication |
J.J.C.M.: 18 (3), 177-183, 2008 |
| Received |
November 6, 2007 |
| Accepted |
May 1, 2008 |
| Abstract |
Mycobacterium tuberculosis complex (MtbC) includes some subspecies such as M. tuberculosis, M. africanum, M. bovis (along with the attenuated M. bovis bacillus Calmette-Guér in[BCG]). Most cases of MtbC infection are caused by M. tuberculosis, but some cases of M. bovis BCG are reported in Japan. We identified 52 strains of MtbC isolated in Kyushu University Hospital between 2004 and 2006. They could not be identified fully by a nitrate reduction test which was one of the conventional methods. Thiophene-2-carboxylic acid hydrazide (TCH) test and the PCR-based methods, however, could distinguish M. bovis BCG strains from M. tuberculosis. Fifty strains (96.2%), including one of a deletion in mpb64 gene, were M. tuberculosis and two (3.8%) were M. bovis BCG. Especially, the PCR-based methods were rapid and useful tool rather than TCH test. Some cases of M. bovis BCG infection due to an intravesical BCG therapy and BCG vaccination are reported. In our study, 2 cases were infected with M. bovis BCG, which were a renal pelvic cancer with intravesical BCG therapy and a chronic granulomatous disease 25 years after BCG vaccination. This study indicates the PCR-based methods are essential to distinguish subspecies from MtbC and necessary for clinical laboratories to report appropriate information for the purpose of clinical therapies, infection controls and epidemiology. |
| Keywords |
BCG, RD, PCR, TCH |
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