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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of Various Media for Rapid Detection of Helicobacter spp. |
| Language |
J |
| AuthorList |
Junko Tomida, Mitsuaki Kashida, Kazuki Oinishi, Ryuichi Endo, Yuji Morita, Yoshiaki Kawamura |
| Affiliation |
Department of Microbiology, Aichi-Gakuin University, School of Pharmacy |
| Publication |
J.J.C.M.: 18 (4), 227-235, 2008 |
| Received |
April 21, 2008 |
| Accepted |
August 1, 2008 |
| Abstract |
The evaluation of commercially available media for Helicobacter species has been carried out mainly by Helicobacter pylori strains. However the reports of Helicobacter cinaedi infection have been increasing, we decided to investigate the performance of various media for growing several Helicobacter species. We evaluated following 8 media with using the type strain of 15 species of the genus Helicobacter, and 14 clinical isolates of H. cinaedi; Sheep blood agar (Nissui), Modified Skirrow agar (Nissui), Modified Skirrow agar EX (Nissui), HP selective agar (Eiken), Columbia Helicobacter pylori agar (BD), Helicobacter agar (Nissui), Vi Helico agar (Eiken), and Pylori agar (Kyokuto). Because the latter three media were containing horse-serum and indicator-dye (tetrazolium salt), the bacterial growth was recognizable at a glance by the violet-blue color on the translucent media. Within these three media, Helicobacter spp. was the best-grown on the Helicobacter agar. To evaluate the total activity of the growing bacteria, ATP assay system was applied. The bacterial activities of the cells collected from Blood agar and Modified Skirrow agar after 5 days culture were dramatically decreased, whereas the cells from the Helicobacter agar were still increased or slightly decreased. In addition, the cells collected from the Helicobacter agar after 5 days culture sustained spiral form, while the majority cells from Blood agar and Modified Skirrow agar changed the form into coccoid shape. From these data, we finally concluded that Helicobacter agar (Nissui) was useful for rapid detection and also sustaining the bacterial activity. |
| Keywords |
Helicobacter cinaedi |
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