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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Solobacterium moorei as a Cause of Sepsis in a Patient with Larynx Cancer: A First Case Report in Japan and Characterization of the Isolate |
| Language |
J |
| AuthorList |
Sanae Mori1), Tomohisa Watari1), Atushi Yasuda1), Sachiko Miura1), Takashi Ogaya1), Munehiro Hatanaka1), Kiyofumi Ohkusu2) |
| Affiliation |
1) Department of Clinical Laboratory, Kitami Red Cross Hospital
2) Department of Microbiology, Gifu University Graduate School of Medicine |
| Publication |
J.J.C.M.: 18 (4), 236-244, 2008 |
| Received |
June 23, 2008 |
| Accepted |
August 18, 2008 |
| Abstract |
Herein we report the first Japanese case of sepsis caused by Solobacterium moorei, an anaerobic, non-sporulated Gram-positive bacillus in a patient with terminal-stage laryngeal cancer. A 60-year-old male was admitted to our hospital with high fever and fatigue. He had a long-lasting medical history and had a subcutaneous neck abscess. A set of blood culture was obtained with the BACTEC Aerobic/92F and Anaerobic/93F resin bottles. After 2 days, Gram-variable polymorphic rods and Prevotella melaninogenica grew from only the anaerobic culture vial. The RapID ANA system indicated that the Gram-variable polymorphic rod was 91.3% likely to be Eggerthella lenta. The API 20A system also showed that it was 94.2% likely to be E. lenta. However, we hesitated to identify it as E. lenta due to the discrepancies between results of our biochemical tests and of those reported in the literature. We therefore performed PCR amplification and DNA sequencing of the 16S rRNA gene of the isolate. A sequence of 1450 bp was found to be 99.6% homologous to the S. moorei type strain sequence (AY044915), indicating that the isolate was a strain of S. moorei, not E. lenta. To further characterize the isolate, we compared it with E. lenta strain (ATCC43055). It was found that they share the same phenotypical characteristics, except the isolate was negative for the catalase and the nitrate reduction tests, and positive for the α-glucosidase test. Thus, it is likely that S. moorei and E. lenta would be differentiated by these three reactions in clinical microbiology laboratory. |
| Keywords |
Solobacterium moorei, 16S rRNA, Eggerthella lenta |
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