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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comparison of the Cobas TaqMan MTB (TaqMan) and COBAS AMPLICOR (COBAS) for the Detection of Mycobacterium tuberculosis |
| Language |
J |
| AuthorList |
Hiroko Yoshida, Tomoshige Matsumoto |
| Affiliation |
Osaka Prefectural Medical Center for Respiratory and Allergic Diseaseas,
3-7-1 Habikino, Habikino-shi, Osaka 583-8588, Japan |
| Publication |
J.J.C.M.: 18 (4), 252-258, 2008 |
| Received |
May 19, 2008 |
| Accepted |
September 22, 2008 |
| Abstract |
The efficiency of the COBAS TaqMan MTB (TaqMan) and COBAS AMPLICOR (COBAS) for the detection of Mycobacterium tuberculosis has been compared. A total of 100 sputum specimens were submitted from 100 patients to our laboratory from May to July 2007. Among them, 70 and 30 were pulmonary tuberculosis and other pulmonary diseases, respectively. Fifty-three were positive and 44 were negative for both methods. The sensitivity of TaqMan and COBAS for smear positive specimens was 91.7% and 88.3%, respectively. Twenty-nine specimens from 30 patients other than tuberculosis were negative by TaqMan, and one specimen was invalid. These 30 specimens were all negative by COBAS. Then, the specificity of two methods was 100%. The agreement ratio of those methods was 97%. The results of those methods highly agreed. TaqMan showed a shorter turn around time than COBAS. TaqMan amplification method will be useful as a routine clinical test for the detection of M. tuberculosis. |
| Keywords |
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