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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Serotype Distribution and Penicillin-Binding Protein Genes of Streptococcus pneumoniae Isolated from the Patients with Acute Otitis Media |
| Language |
J |
| AuthorList |
Iku Kurokawa1), Junko Masaki1), Naoko Chiba2), Kimiko Ubukata2) |
| Affiliation |
1) Department of Laboratory Medicine, Tohoku Rosai Hospital, Japan Labor Health & Welfare Organization, 4-3-21 Dainohara, Aoba-ku, Sendai-shi, Miyagi 981-8563, Japan
2) Kitasato Institute for Life Science & Graduate School of Infection Control Sciences, Kitasato University |
| Publication |
J.J.C.M.: 19 (1), 5-11, 2009 |
| Received |
August 12, 2008 |
| Accepted |
December 24, 2008 |
| Abstract |
We studied 426 acute otitis media (AOM) cases for the period from August 2006 to May 2007. Specimens were collected by myringotomy, and 190 (41.1%) strains were isolated, in which 75 isolates were identified as Streptococcus pneumoniae. Abnormal PBP genes of pbp1a, pbp2x, and pbp2b were identified by PCR as follows: (i) genotype (g) PSSP with normal PBP gene (8.0%), (ii) gPISP with an abnormal pbp2x gene (29.3%), (iii) gPISP with an abnormal pbp2b gene (5.3%), (iv) gPISP with an abnormal pbp2x+2b gene (5.3%), (v) gPISP with an abnormal pbp1a+2x gene (8.0%), and (vi) gPRSP with an abnormal pbp1a+2b+2x (44.0%) gene. Pneumococcal serotype of isolates were 3 (25.3%), 19F (18.7%), 6A (16.0%), 14 (9.3%), and 23F (9.3%). The MICs value of PCG in serotype 3 strains with pbp2x (mucoid type) or serotype 14 strains with pbp1a+2x demonstrating small colonies were considered to be ≤0.06 μg/ml by dilution antimicrobial susceptibility methods commonly used in our daily laboratory work. It is concluded that we need to re-examine the conditions of bacterial inoculum size and the medium at the time of measurement of susceptibility. |
| Keywords |
Streptococcus pneumoniae, serotype, penicillin-binding protein genes, acute otitis media |
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