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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of the COBAS TaqMan MTB Assay in Detection of Mycobacterium tuberculosis Complex |
| Language |
J |
| AuthorList |
Norihiko Akamatsu1), Katsunori Yanagihara1), Junichi Matsuda1), Takayoshi Kiya1), Kazuyuki Sugahara1), Maiko Motoshima1), Maiko Nakamura1), Tsuyosi Unno2), Yasuaki Yamada3), Shimeru Kamihira3) |
| Affiliation |
1) Department of Clinical Laboratory, Nagasaki University Hospital of Medical and Dentistry
2) Roche Diagnostics K.K.
3) Department of Laboratory Medicine Nagasaki University Graduate School of Biomedical Sciences |
| Publication |
J.J.C.M.: 19 (2), 84-89, 2009 |
| Received |
July 4, 2008 |
| Accepted |
January 28, 2009 |
| Abstract |
We evaluated the COBAS TaqMan MTB assay (TaqMan) in comparison with the COBAS AMPLICOR MTB assay (AMPLICOR) for detection of Mycobacterium tuberculosis complex from clinical specimens. Both assays indicated the same data in 100 from 109 specimens. The four samples were TaqMan-positive and AMPLICOR-negative. In addition, four of the rest five specimens occurred in PCR-inhibition of AMPLICOR, but not in TaqMan. six specimens were escaped from PCR-inhibition in TaqMan in 9 specimens with PCR-inhibition of AMPLICOR. These results indicate TaqMan is better than AMPLICOR is a very useful for clinical diagnosis in future. |
| Keywords |
TaqMan |
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