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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of the Gram Stainability Newly Developed in Small Automatic Staining Machine, GS-20A |
| Language |
J |
| AuthorList |
Yoshiko Atsukawa1), Sayoko Kawakami1), Shinobu Ishigaki1), Takashi Tanaka1), Miwa Asahara1), Yasuo Ono2), Yukihisa Miyazawa1) |
| Affiliation |
1) Department of Central Laboratory, Teikyo University Hospital
2) Department of Microbiology and Immunology, Teikyo University School of Medicine |
| Publication |
J.J.C.M.: 19 (3), 142-147, 2009 |
| Received |
March 13, 2009 |
| Accepted |
May 7, 2009 |
| Abstract |
The stainability for Gram staining by a newly developed small automatic staining machine (GS-20A) was evaluated. Though, the stainability for Gram stain by GS-20A was excellent (96.61%; 2,652/2,745), defective staining rate of the nuclei of the cells, such as white blood cell or epithelial cells, defective staining rate of the bacteria were 0.84% (23/2,745), and 2.26% (62/2,745), respectively, and pigment granule deposition was seen in 0.29% (8/2,745). The defective staining rate of the Gram positive bacteria which should have been dyed by Gram positive were 1.71% (47/2,745) for cocci and 0.62% (17/2,745) for bacilli, respectively. To conduct appropriate Gram staining, samples with high contents of sputum, pus, or blood constituents should be smeared evenly and thinly onto slides in sample preparation, and the length of time for bleaching should be kept short just after reagent replacement. This staining machine allows different time settings for various purposes, and is also suitable for staining a large number of prepared samples. This machine is very effective for rapid microscopic examination of smear samples from sites of infection. |
| Keywords |
GS-20A |
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