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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Prevalence and Combined Effects of Antibacterial Agents against Clinically Isolated Multi-Drug Resistant Pseudomonas aeruginosa Strains |
| Language |
J |
| AuthorList |
Hitoshi Miyamoto, Shinobu Murakami, Mitsuharu Murase |
| Affiliation |
Division of Medical Technology, Ehime University Hospital |
| Publication |
J.J.C.M.: 19 (3), 157-162, 2009 |
| Received |
February 16, 2009 |
| Accepted |
May 27, 2009 |
| Abstract |
The effectiveness of antibacterial agents against 22 strains of clinically isolated multi-drug resistant Pseudomonas aeruginosa (MDRP) was measured by the micro-dilution method. The all strains produced Metallo-β-lactamase (MBL), and the IMP-1 gene was detected by the polymerase chain reaction (PCR). The combined effects of antibacterial agents were tested against 22 strains of MDRP selected by the Breakpoint Checkerboard Plate method after evaluation by the fractional inhibitory concentration (FIC) index. The incidence of MDRP was ranged from 0.9 to 2.5% in the last 5 years. Twelve MBL-positive strains were detected in genes for the IMP-1 group by PCR. Six of the other 10 strains were susceptible for CAZ. MDRP showed good susceptibilities to the combination of aztreonam and amikacin in 59.1%. Further studies are needed to find a new effective combination of antimicrobial agents to multi-drug resistant strains. |
| Keywords |
multi-drug resistant Pseudomonas aeruginosa, Breakpoint Checkerboard Plate, Metallo-β-lactamase, ceftazidime |
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