 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
New Screening Medium Developed for Detecting Multidrug-resistant Pseudomonas aeruginosa |
| Language |
J |
| AuthorList |
Kumiko Kawamura |
| Affiliation |
Department of Medical Technology, Nagoya University School of Health Science |
| Publication |
J.J.C.M.: 19 (4), 220-229, 2009 |
| Received |
April 1, 2009 |
| Accepted |
October 4, 2009 |
| Abstract |
Multidrug-resistant Pseudomonas aeruginosa (MDRP) is responsible for severe nosocomial infections, and rapid and accurate methods to detect such strains are needed. We developed a new MDRP screening medium by incorporation of three antimicrobial agents, amikacin (AMK), imipenem/cilastatin (IPM) and ciprofloxacin (CPFX), into selective mediums for the detection of Pseudomonas species such as CHROMagarTM Pseudomonas (CHROMagar) and NAC agar. The optimal concentrations of the three antimicrobial agents added to the selective mediums were determined using a receiver operating characteristic (ROC) curve. As the result, three kinds of CHROMagar with 1 μmg/ml of AMK, 4, 6 or 8 μg/ml of IPM and 1 μg/ml of CPFX (sensitivity of 88.9% and specificity of 96.3-100%, respectively), and two kinds of NAC agar with 12 μg/ml of AMK, 12 or 24 μg/ml of IPM and 1 μg/ml of CPFX (sensitivity of 96.3-100%, respectively, and specificity of 92.6%), were selected as the optimal conditions for the detection of clinical MDRP isolates. The MDRP screening mediums based on the five conditions selected by ROC analysis were evaluated, and CHROMagar with 1 μmg/ml of AMK, 4 and 6 μg/ml of IPM and 1 μg/ml of CPFX showed the best detection limit (103 cfu/ml), sensitivity and specificity among the five conditions. Additionally, the stability of the medium was confirmed until four weeks at 4°C. The MDRP screening medium is a simple, rapid and cost-effective method compared to the conventional methods described in the literature. Thus, the use of the MDRP screening medium would make it possible to detect efficiently MDRP isolates in clinical material. |
| Keywords |
MDRP |
|
