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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Morphological and Biochemical Identification of Cigar-Shaped Clostridia |
| Language |
J |
| AuthorList |
Yasushi Daimon1), Kaori Tanaka2), Kunitomo Watanabe2) |
| Affiliation |
1) Clinical Laboratory, Kyoto Prefectural Yosanoumi Hospital
2) Division of Anaerobe Research, Life Science Research Center, Gifu University |
| Publication |
J.J.C.M.: 20 (1), 9-19, 2010 |
| Received |
June 19, 2009 |
| Accepted |
November 2, 2009 |
| Abstract |
Clostridium clostridioforme has been known to be phenotypically heterogeneous species with cigar-shaped morphology. Recent taxonomic studies have shown that C. clostridioforme represents five distinct species in the C. clostridioforme group: Clostridium hathewayi, C. clostridioforme, Clostridium bolteae, Clostridium citroniae, and Clostridium aldenense. A total of 60 strains isolated in our laboratory as the cigar-shaped clostridia were identified through end-product analysis and 16S rRNA sequencing as follows: C. hathewayi (16 strains), C. clostridioforme (10), C. bolteae (11), C. citroniae (6), C. aldenense (5), and C. symbiosum (12). Biochemical characteristics for those isolates were investigated using commercially available identification kits. C. citroniae and C. aldenense were distinguished from each other by the results for rhamnose, β-glucuronidase, β-galactocidase (C. citroniae : +, +, -, C. aldenense : -, -, +, respectively), although both of them were indole positive. C. hathewayi was differentiated from other species in C. clostridioforme group by the positive reaction of cellobiose, α-arabinosidase, N-acetylglucosaminidase. It was difficult to distinguish between C. clostridioforme and C. bolteae by phenotypic characteristics. C. symbiosum was distinguishable from C. clostridioforme group in colonial size. |
| Keywords |
Clostridium clostridioforme, Clostridium bolteae, Clostridium hathewayi, Clostridium citroniae, Clostridium aldenense |
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