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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Susceptibility and Molecular Epidemiology of IMP-19 and IMP-1 Metallo-β-lactamase Producing Acinetobacter baumannii Isolated in a University Hospital during 4 Years |
| Language |
J |
| AuthorList |
Tomomi Mochimaru1), Yujiro Uchida2), Yuiko Morokuma1), Satoko Yogata1), Makiko Kiyosuke1), Masako Fujise1), Toshiharu Tsutsui1), Fujiko Eto1), Nobuyuki Shimono3), Yuzo Kayamori1), Dongchon Kang1,2) |
| Affiliation |
1) Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
2) Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences
2) Faculty of Medicine and Biosystemic Science, Graduate School of Medical Science, Kyushu University |
| Publication |
J.J.C.M.: 20 (1), 28-37, 2010 |
| Received |
July 28, 2009 |
| Accepted |
November 20, 2009 |
| Abstract |
The isolation ratio of MBL producing Acinetobacter baumannii was 67.6% among all MBL producing bacteria isolated in our hospital from 2004 to 2007, although the most popular MBL producing bacteria were Pseudomonas aeruginosa in Japan. Therefore we studied on molecular epidemiology for the MBL producing A. baumannii and clinical characteristics of patients with the isolates. In 49 MBL producing A. baumannii, 43 (87.8%) of IMP-19 and 6 (12.2%) of IMP-1 were detected by PCR and DNA sequence analysis. Antimicrobial tests showed a MIC50 value of Imipenem (IPM) for IMP-19 isolates was higher than Meropenem (MEPM), while the value of MEPM for IMP-1 isolates was higher than IPM. MIC50 values of Ampicillin/Sulbactam for IMP-19 and IMP-1 were 4 μg/ml and 8 μg/ml, respectively, and the values of Cefoperazone/Sulbactam (CPZ/SBT) for IMP-19 and IMP-1 were 2 μg/ml and 4 μg/ml, respectively. Statistical analyses revealed that the risk factors for infections with MBL producing A. baumannii were associated with severe underlying diseases or immunosuppressive state with the administration of steroids or antifungals, the history of solid organ transplantation and the stay in ICU. Pulsed-field gel electrophoresis analysis demonstrated 19 groups among 49 isolates, which supposed to exist nosocomial infections in our hospital. The ratio of the MBL producing A. baumannii reduced after improvement of infection controls for patients with the isolates, which indicated that the coordination between a hospital laboratory and an infection control team were the most important for infection controls in a hospital. |
| Keywords |
Acinetobacter baumannii, IMP-1, IMP-19 |
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