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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Rapid and Accurate Detection of Mycobacterium kansasii in Sputum by Second-Step PCR Following COBAS AMPLICOR PCR |
| Language |
J |
| AuthorList |
Norihiko Akamatsu1), Katsunori Yanagihara1), Junichi Matsuda1), Takayoshi Kiya1), Maiko Yamada1), Sayaka Mori1), Yasuaki Yamada1,3), Shigeru Kohno2), Shimeru Kamihira1,3) |
| Affiliation |
1) Department of Clinical Laboratory, Nagasaki University Hospital of Medical and Dentistry
2) Second Department of Internal Medicine, Nagasaki University Hospital of Medical and Dentistry
3) Department of Laboratory Medicine Nagasaki University Graduate School of Biomedical Sciences |
| Publication |
J.J.C.M.: 20 (2), 125-129, 2010 |
| Received |
October 15, 2009 |
| Accepted |
April 6, 2010 |
| Abstract |
We evaluated a rapid and accurate method for detecting M. kansasii in sputum. The COBAS AMPLICOR PCR was carried out on 8 strains of Mycobacterium (2 each of M. kansasii, M. tuberculosis, M. avium, and M. intracellulare) and a total of 35 Mycobacterium-positive sputa (M. kansasii, n=7; M. tuberculosis, n=7; M. avium, n=7; M. intracellulare, n=7; M. chelonae, n=2; M. abscessus, n=2; M. gordonae, n=2; and M. fortuitum, n=1). Both isolated M. kansasii strains and all M. kansasii-positive sputum samples (n=7) were detected by second-step PCR. Moreover, there were no cross reactions to other species. Direct sequencing of the second-step PCR products in M. kansasii-positive sputa showed complete homology with M. kansasii (Accession no. AJ536035) in a Basic Local Alignment Search Tool (BLAST). These findings indicate that this method is rapid, accurate and can be applied to the detection of M. kansasii in a clinical setting. |
| Keywords |
Mycobacterium kansasii, PCR |
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