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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Latex Agglutination Kit Using Dryspot Staphytect Plus for Staphylococcus aureus Detection: Effects of Culture Medium and Time |
| Language |
J |
| AuthorList |
Yoko Tazawa1), Hiromi Sasaki1), Yukie Furuhata1), Yuji Kikuchi1), Hajime Horiuchi1), Ryoichi Kubo2), Hideaki Hanaki3), Jun Okada4) |
| Affiliation |
1) Clinical Laboratory, NTT Medical Center Tokyo
2) Life Science Department, Kanto Chemical Co., Inc.
3) Kitasato Research Center for Anti-infection Drugs, Kitasato Medical University
4) Faculty of Sports and Health Science, Daito Bunka University |
| Publication |
J.J.C.M.: 20 (2), 134-137, 2010 |
| Received |
May 14, 2008 |
| Accepted |
January 25, 2010 |
| Abstract |
As polysaccharides are present on the cell wall of methicillin-resistant Staphylococcus aureus (MRSA), this bacterium may yield false negatives on latex agglutination tests which use conventional reagents that detect bound coagulase or protein A. Dryspot Staphytect Plus has recently become commercially available as a latex reagent that can recognize both coagulase and protein A, as well as capsular polysaccharides, as antigens. We obtained isolates from 40 MRSA strains are known to have negative reactivity to the conventional latex reagent PS LATEX 'Eiken,' and compared their reactivity to PS LATEX with Dryspot after 18, 24, and 48 h incubation in sheep blood agar or in CHROMagar MRSA. For reactivity of PS LATEX alone, all 40 strains were confirmed to be negative regardless of culture medium and incubation time. For PS LATEX with Dryspot reactivity, three of the 40 strains were positive in both the 18- and 24-h blood agar cultures, while 15 and 23 strains were positive in the 18- and 24-h CHROMagar cultures, respectively, for a total of 38 positive strains. These results suggest that Dryspot is a useful reagent for detecting MRSA strains that cannot be detected with conventional latex reagents and is effective for cultures incubated over 24 h in CHROMagar. |
| Keywords |
MRSA |
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