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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Analysis of Resistance Genes in Fluoroquinolone High-resistant and Mucoid Type Streptococcus pyogenes Isolated from Blood Culture |
| Language |
J |
| AuthorList |
Ryo Kawaguchi1), Hirosuke Matuo1), Kimiko Ubukata2) |
| Affiliation |
1) Department of Clinical Laboratory, Sasebo Chuo Hospital
2) Laboratory of Molecular Epidemiology for Infectious Agents, Kitasato University |
| Publication |
J.J.C.M.: 20 (2), 138-142, 2010 |
| Received |
January 22, 2010 |
| Accepted |
April 19, 2010 |
| Abstract |
Mucoid type Streptococcus pyogenes was detected in a blood culture obtained from a female with cellulitis. She had systemic lupus erythematosus. The isolate of S. pyogenes was highly resistant to fluoroquinolone. DNA amplification and sequencing revealed that the main mechanism of resistance was mediated by point mutations in the quinolone resistance-determining region of gerA, which encodes DNA gyrase, and parC, which encodes topoisomerase. The isolate presented a point mutation in gyrA, predicting that serine-81 was changed to phenylalanine-81 and in parC, thereby predicting that serine-79 changed to phenylalanine-79. It is feared that fluoroquinolone-resistant S. pyogenes might increase due recent increases in the dosages of fluoroquinolone that are being administered to adults. |
| Keywords |
Streptococcus pyogenes, fluoroquinolone resistance, gerA gene, parC gene |
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