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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Practical Evaluation of 2% Ogawa Medium in a New Screw-capped Plastic Bottle |
| Language |
J |
| AuthorList |
Akio Aono1), Yuka Azuma1), Tatsuji Kuwahara1), Satoshi Mitarai2) |
| Affiliation |
1) Department of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis Association
2) Bacteriology division, Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association |
| Publication |
J.J.C.M.: 20 (3), 177-181, 2010 |
| Received |
March 31, 2010 |
| Accepted |
July 12, 2010 |
| Abstract |
The 2% Ogawa medium for the culture of mycobacteria, which is commercially available in Japan, is considered to be inappropriate with respect to the bio-safety, because it is normally prepared in a glass tube with a rubber cap. It will break in case of accident, and the cap will come off easily. Recently, the 2% Ogawa medium in newly designed plastic bottle (plastic Ogawa) with screw cap was evaluated for its performance of initial isolation of mycobacterial strains. The 2% Ogawa media in the conventional glass tube (glass Ogawa) and the plastic Ogawa were compared for their growth supporting power using the clinical specimens. A total of 231 sputum specimens from 101 patients were subjected to the culture examination with the plastic and glass Ogawa media. The plastic and glass Ogawa recovered 169 and 172 of mycobacterial strains, respectively. The overall agreement of the number of colonies on these media was 93.3%, and the turn-around time was comparable in 90.7%. The discrepancy between two methods was due to the fewer colonies observed on the plastic Ogawa, which was kept in slanted position with inappropriate rack. The plastic Ogawa medium was considered to be equivalent to the glass one, and to be safe. It must be recommended that the plastic bottle can replace the glass Ogawa. |
| Keywords |
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