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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Positive Gram's Stain of Blood Culture without Positive Signals on BACTEC9050 |
| Language |
J |
| AuthorList |
Akiko Furutani1), Satomi Yoshida1), Aya Kubo1), Maiko Yamashita1), Tatsuaki Itoh1), Michio Suzuki2), Koichi Imaoka2), Kiyofumi Ohkusu3) |
| Affiliation |
1) Department of Clinical Laboratory, Sasebo Kyosai Hospital
2) Department of Veterinary Science, National Institute of Infectious Diseases
3) Department of Microbiology, Gifu University Graduate School Medicine |
| Publication |
J.J.C.M.: 20 (3), 182-187, 2010 |
| Received |
February 1, 2010 |
| Accepted |
April 30, 2010 |
| Abstract |
Capnocytophaga canimorsus is capnophilic, slow-growing, filamentous, facultative aerobic, Gram-negative bacteria and is found in the oral cavity of dogs and cats. C. canimrosus sometimes causes septicemia, endocarditis and meningitis in people after dog or cat bite and fatality rate due to septicemia is estimated up to 30%. We encountered the case of septicemia due to C. canimrosus infection by a pet dog bite. After 3 days blood culture, any positive signals were not observed on BACTEC9050, however we performed Gram's stain and PCR of blood samples from culture bottles and obtained positive staining and apecific amplification of 16S rRNA gene of C. canimrosus. Thereafter, isolated bacterial strains from subcultures of blood culture and wound tissues were also determined as C. canimrosus by PCR, genome sequencing analysis and biochemical test. This study suggests that early detection of bacteria by Gram's stain and PCR of the blood culture is helpful for the determination of primary causative organism and treatment of septic patient, even if positive signal is not observed on automated blood culture microbial detection system. |
| Keywords |
Capnocytophaga canimorsus, PCR |
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