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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Molecular Analyses of IND-Type β-Lactamase Genes from Recent Clinical Chryseobacterium indologenes Isolates |
| Language |
J |
| AuthorList |
Mika Nagata1), Takehisa Matsumoto2), Miki Matsubara2), Eriko Kasuga1,2), Kozue Oana1,3), Yoshiyuki Kawakami1,3) |
| Affiliation |
1) Division of Infection Control and Microbiological Regulation in Health Sciences, Shinshu University Graduate School of Medicine
2) Department of Laboratory Medicine, Shinshu University Hospital
3) Division of Clinical Microbiology, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine |
| Publication |
J.J.C.M.: 20 (3), 203-206, 2010 |
| Received |
March 31, 2010 |
| Accepted |
July 12, 2010 |
| Abstract |
Chryseobacterium indologenes is known to demonstrate a decreased susceptibility to most β-lactam agents including carbapenems, due to the production of chromosome-encoded IND-type metallo-β-lactamase (MBL). Molecular and epidemiological analysis of the 13 recent clinical isolates and an ATCC29897T, the type strain of C. indologenes, disclosed that they were classified into 6 types consisting of 3 IND-2, 2 IND-2a, and 1 uncategorized IND types. The 99% homology (241aa/243aa) of the amino acid sequences was observed between the uncategorized IND and the IND-2 types. However, in the subsequent analysis of the uncategorized IND type, the amino acid substitutions of W167R and T208M were noted in comparison with the IND-2 gene, which we designated as IND-15. Only the strain possessing the IND-15 type gene exhibited susceptible against imipenem and β-lactams with β-lactamase inhibitors. It should be noted that some strains reveal to be sensitive to carbapenems notwithstanding the possession of resistance genes. |
| Keywords |
Chryseobacterium indologenes, metallo-β-lactamase, IND |
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