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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Investigation on the Relation of Biofilm Formation to Capsular Types and Surface Proteins in Standard Strains of Klebsiella |
| Language |
J |
| AuthorList |
Yuka Ishihara1,2), Michio Ohta1,2) |
| Affiliation |
1) School of Nursing, Sugiyama Jogakuen University
2) Department of Bacteriology, Graduate School of Medicine, Nagoya University |
| Publication |
J.J.C.M.: 20 (4), 225-232, 2010 |
| Received |
June 2, 2010 |
| Accepted |
August 9, 2010 |
| Abstract |
Cell surface capsular polysaccharide (K antigen) and pili are known as major virulence factors of Klebsiella. Previous reports suggested that these components are also involved in biofilm formation. The present study was undertaken for revealing the relationship between degree of biofilm formation and capsular types as well as colony viscosity. A total of 76 serotype standard strains were cultured at 37°C and 25°C for biofilm formation on polystyrene surface and the effect of proteinase K treatment was measured. Strains K8, K17, K27 and K37 formed high level of biofilm and their colonies were non-hypermucoviscosity type. On the other hand, strains K1, K2 and K3 belonged to a group of very poor biofilm formation and their colonies were hypermucoviscosity type, suggesting that mucoviscosity of colonies was unrelated, or inversely correlated with biofilm formation ability of Klebsiella. Biofilm formation of K8, K27 and K79 strains at 37°C and of K27 at 25°C was greatly reduced by proteinase K treatment. Proteinase K-sensitive surface proteins therefore were involved in the biofilm formation of these strains. The high level biofilm formation of K37 strain, however, was not affected by proteinase K treatment. It is therefore likely that the capsular polysaccharide is a major constituent of the K 37 biofilm. Taken together, biofilm formation level of Klebsiella varies in K type strains and the biofilm includes proteinase K-sensitive and-resistant types. |
| Keywords |
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