Journal

The Journal of the Japanese Society for Clinical Microbiology

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[Vol.20 No.4 contents]
Japanese / English

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Article in Japanese

ArticleTitle First Clinical Isolate of Paracoccus yeei in Japan
Language J
AuthorList Yoko Tazawa1), Hiromi Sasaki1), Yukie Furuhata1), Yuji Kikuchi1), Hajime Horiuchi1), Kiyofumi Ohkusu2)
Affiliation 1) Clinical Laboratory, NTT Medical Center Tokyo
2) Department of Microbiology, Gifu University Graduate School of Medicine
Publication J.J.C.M.: 20 (4), 233-238, 2010
Received April 9, 2010
Accepted August 30, 2010
Abstract Paracoccus yeei was registered as a new species in 2003, and the first Japanese patient with P. yeei is presented herein. The patient was a 74-year-old man admitted in February 2008, diagnosed with pancreatic head cancer, and scheduled to undergo pancreatoduodenectomy. Findings indicating biliary tract infection were absent preoperatively, and the patient's bile sample was sent to the laboratory for screening purposes. A smear test showed Gram-positive cocci and Moraxella catarrharis-like Gram-negative diplococci, while isolation culture identified not only MSSA, but also mucoid colonies. These colonies were catalase-positive, oxidase-positive, Gram-negative cocci; automatic identification and sensitivity analysis indicated Moraxella sp., but colony morphologies did not match. The colonies could not be identified using a simple kit. Subsequently, a genetic test was conducted, and based on 99.8% homology of the base sequence of 16S rDNA, it was concluded that P. yeei had been isolated for the first time in Japan. When strains that cannot be identified by identification equipment or kits are detected, it is important to promptly share information about isolated bacteria with patients and healthcare professionals and employ such techniques as genetic identification.
Keywords Paracoccus yeei, 16S rRNA, Moraxella catarrharis
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